Work Packages
WP1 External controllers
- investigate external controllers implementing various frameworks for modelling, state estimation and model predictive control
- study their robustness and adaptability on a challenging control problem: balancing a toggle switch in E. coli and S. cerevisiae
WP2 Embedded controllers
- Design new tools and methods for analysis and design of controllers in the presence of intracellular noise
- Develop new strategies for designing robust embedded controllers that have a small footprint and low metabolic loads
- Develop new theory and methods for building biological insulators that enable the modular connection of embedded biological systems in a noisy environment
- Develop a theory for nonlinear system realization using biological components
- Design and build embedded controllers for the E. coli and yeast toggle switches
WP3 Multicellular Control
- The key goal of this work package is to study how to deploy control across cell populations to develop and demonstrate an integrated approach to create a synthetically engineered consortium where subpopulations of genetically engineered cells can respond to external reference inputs and self-regulate each other to exhibit a desired function
WP4 Automated microfluidics platform for quantitative modelling of biological processes
- To design new model identification methods and tools for closed-loop modelling
- To design new model based optimal experimental methods and tools for closed-loop model identification
- To build an integrated platform for biomodel identification (BMID), optimal design of experiments (OED) and rapid prototyping of synthetic biology systems
- To test and validate the performance of the rapid prototyping platform in three case study applications
WP5 Experimental applications in E. coli
- External control of plasmid copy number and growth rate in E. coli cells
- Multicellular feedback control of protein concentration in E. coli cells
WP6 Taking control of the cell cycle in yeast
- Demonstrate that external and embedded control of gene expression can be used to drive the cell cycle in yeast
WP7 Management
- To manage the project according to the approved plan, support the Participants in achieving the objectives, complete the milestones in time and convey the deliverables
- To ensure that the required reports are made and delivered in a timely manner according to quality assurance standards and homogeneity
- To monitor, track and control deviations due to progress, cost, financial and scheduling change
- Manage the processes to properly protect intellectual property, including confidential information and patents
WP8 Communication, dissemination and exploitation of the results
- Communication to stakeholders and the general public will be organized through workshops, summer schools, meetings, participation in media activities and via e-communication tools as part of the central interaction website
- Dissemination and exploitation of the results